Manual of Practical Biochemistry, 5th Edition
Rafi MD
Price
475
ISBN
9788197424977
Language
English
Pages
244
Format
Paperback
Dimensions
180 x 240 mm
Year of Publishing
2025
Territorial Rights
World
Imprint
Universities Press

This book provides a concise introduction to the qualitative and quantitative analysis of substances of biochemical importance for undergraduate medical students. It covers all the skill-based aspects of the CBME curriculum for biochemistry.

Salient features
  • Maps the contents of the book to the CBME curriculum for easy reference
  • Topics such as urinary screening tests for inborn errors of metabolism, energy content of various food items, significance of glycaemic index, and saturated, unsaturated and trans-fatty acids in food are included in this edition
  • Tabulations summarising biochemical tests, procedures and outcomes included appropriately to
  • make it easy for students to obtain information at a glance
  • Exercises comprising MCQs, viva voce questions, and questions based on problem-solving skills and interpretation of laboratory data of common clinical conditions are updated

Rafi MD, Dean and Medical Director of Surabhi Institute of Medical Sciences, Siddipet, obtained his MBBS degree from Kakatiya Medical College, Warangal, and MD from Gandhi Medical College, Hyderabad. He is known for his passion for maximising the learning experience for his students through innovative teaching methods. Dr Rafi has several research publications to his credit in journals of repute.

1. Safety in the Laboratory
Precautions
First Aid in the Laboratory

QUALITATIVE EXPERIMENTS
2. Reactions of Non-protein Nitrogenous (NPN) Substances
Urea
Uric Acid
Creatinine
Identification of unknown NPN substances
3. Analysis of Urine
Physical Examination
Microscopic Examination
Chemical Analysis
4. Analysis of Abnormal Constituents of Urine
Glucose
Ketone Bodies
Proteins
Blood
Bile Salts
Bile Pigments
Identification of unknown abnormal constituents in urine

QUANTITATIVE EXPERIMENTS
5. Clinical Biochemistry Laboratory: Instrumentation and Techniques
Glassware and Instruments Commonly Used in the Laboratory
Solutions
Sample Collection and Handling
Colorimetry
Spectrophotometer
Autoanalyser
Flame Photometer
Fluorimeter
Dry Chemistry Systems
ABG Analysis
Immunodiffusion
Quality Control
6. Blood Glucose
Sample Collection
Determination of Glucose in Body Fluids
7. Glucose Tolerance Test
Indications for GTT
Glucose Tolerance Curve
8. Blood Urea and BUN
Methods of Blood Urea Estimation
Urinary Urea and Urea Clearance
9. Serum and Urinary Creatinine and Creatinine Clearance
Creatine and Creatinine
Creatinine Clearance
10. Uric Acid
Formation and Excretion of Uric Acid
Methods of Estimation
11. Serum Total Proteins and Albumin–Globulin Ratio
12. Cholesterol and Lipid Profile
13. Bilirubin
Serum Bilirubin
14. Aminotransferases (AST and ALT or SGOT and SGPT)
15. Alkaline and Acid Phosphatases (ALP and ACP)
Estimation of Alkaline Phosphatase
Estimation of Acid Phosphatase
16. Creatine Kinase and Its Isoenzymes
17. Amylase and Lipase
Estimation of Serum Amylase
Estimation of Serum Lipase
18. Calcium and Phosphorus
19. Serum Electrolytes
Electrolyte Analysis by Ion Selective Electrode
20. Quantitative Analysis of CSF
21. Electrophoresis
Types of Electrophoresis
22. Chromatography
23. Radioimmunoassay (RIA) and Enzyme-linked Immunosorbant Assay
RadioImmunoassay (RIA)
Enzyme-linked Immunosorbant Assay (ELISA)
24. Estimation of pH and Preparation of Buffers
Determination of pH Using a pH Meter
Buffers
25. Biomedical Waste Management
Segregation of Waste
Storage of Waste
Transportation of Waste
Treatment and Disposal of Waste
Safety Measures
26. DNA Isolation and Quantification
Basic Steps of DNA Isolation
Quantification of DNA
27. Biochemical Tests for Common Clinical Conditions
Diabetes Mellitus
Dyslipidemia
Myocardial Infarction
Renal Failure
Gout
Proteinuria
Nephrotic Syndrome
Edema
Jaundice
Liver Disorders
Pancreatitis
Acid-Base Disorders
Thyroid Disorders
Vitamins and Minerals
28. Urinary Screening for Inborn Errors of Metabolism
29. Energy Content of Various Foods and the Significance of Glycemic Index
Energy Requirements in Humans
Energy Content of Foods
Glycemic Index
30. Saturated, Unsaturated and Trans-Fatty Acids in Foods
Saturated Fatty Acids
Unsaturated Fatty Acids
Biomedical Importance of PUFA
Trans-fats

APPENDICES
I. Practical Biochemistry Question Bank
Qualitative Experiments
Quantitative Experiments
II. Multiple-choice Questions (MCQs) in Practical Biochemistry
III. Problem-solving Exercises, Interpretation of Laboratory Data and
Case Studies in Practical Biochemistry
IV. Test Procedures of Qualitative and Quantitative Experiments in a Nutshell
Qualitative Test Procedures (in alphabetical order)
Quantitative Test Procedures
Spotters and Charts