This book introduces some of the most widely used experimental procedures in biochemistry for students of MBBS and other allied sciences. In this new edition, all the chapters have been thoroughly revised to conform to the Competency-Based Medical Education (CBME) curriculum.

Salient features

• A wide range of qualitative analysis and quantitative estimations and measurements in biochemistry explained with emphasis on principles, procedures, observations and clinical interpretations

• Theory of the underlying principles and procedures included so that students are able to appreciate the biochemical basis of the demonstrated experiments/techniques

• Covers all the competencies prescribed in the CBME curriculum

• Several new topics or expanded treatment of topics such as electrophoresis, chromatography, estimation of pH and preparation of buffers, biomedical waste management, isolation and quantification of DNA, and biochemical tests for common clinical conditions included in this edition

• Features viva questions commonly encountered in practical examinations

• Includes MCQs, problem-based exercises, questions relating to interpretation of laboratory data and case studies to aid students in their preparations for competitive examinations

Rafi MD, Dean and Medical Director of Surabhi Institute of Medical Sciences, Siddipet, obtained his MBBS degree from Kakatiya Medical College, Warangal, and MD from Gandhi Medical College, Hyderabad. He is well-known for his passion for maximising the learning experience for his students through innovative teaching methods. Dr Rafi has several research publications to his credit in journals of repute.

Preface to the Third Edition
Preface to the First Edition

QUALITATIVE EXPERIMENTS
1. Safety in the Laboratory

2. Reactions of Carbohydrates
Reactions of Glucose
Reactions of Fructose
Reactions of Maltose
Reactions of Lactose
Reactions of Sucrose
Identification of Unknown Carbohydrate

3. Reactions of Lipids
Reactions of Triacylglycerols
Reactions of Cholesterol

4. Reactions of Proteins
Precipitation Reactions of Proteins
Colour Reactions of Proteins
Reactions of Albumin
Reactions of Casein
Reactions of Gelatin
Reactions of Peptone
Identification of Unknown Protein

5. Reactions of Non-protein Nitrogenous (NPN) Substances
Urea
Uric Acid
Creatinine
Identification of Unknown NPN Substance

6. Identification of Unknown Substance of Biochemical Importance
Carbohydrates, Proteins and NPN Substances

7. Analysis of Urine
Physical Examination
Microscopic Examination
Chemical Analysis

8. Analysis of Abnormal Constituents in Urine
Glucose
Ketone Bodies
Proteins
Blood
Bile Salts
Bile Pigments
Identification of Unknown Abnormal Constituents in Urine

9. Qualitative Analysis of Saliva

10. Qualitative Analysis of Gastric Juice

11. Qualitative Analysis of Bile

12. Qualitative Analysis of Milk

13. Food Analysis

QUANTITATIVE EXPERIMENTS

14. Introduction to Clinical Biochemistry
Glassware and Instruments Used in the Laboratory
Solutions
Sample Collection and Handling
Colorimetry
ABG Analysis
Immunodiffusion
Quality Control

15. Blood Glucose

16. Glucose Tolerance Test

17. Blood Urea and BUN

18. Serum and Urinary Creatinine

19. Uric Acid

20. Serum Total Proteins and Albumin–Globulin Ratio

21. Cholesterol and Lipid Profile

22. Bilirubin

23. Aminotransferases (AST and ALT or SGOT and SGPT)

24. Alkaline and Acid Phosphatases (ALP and ACP)

25. Creatine Kinase and Its Isoenzymes

26. Amylase and Lipase

27. Calcium and Phosphorus

28. Serum Electrolytes

29. Quantitative Analysis of CSF

30. Electrophoresis
Paper Electrophoresis
Gel Electrophoresis
Isoelectric Focussing
Capillary Electrophoresis

31. Chromatography
Thin Layer Chromatography (TLC)
Gas–Liquid Chromatography
Ion Exchange Chromatography
Gel Exclusion (Permeation) Chromatography
Affinity Chromatography
High Performance (Pressure) Liquid Chromatography (HPLC)

32. Spectroscopic Examination of Hemoglobin and Its Derivatives

33. Radioimmunoassay (RIA) and Enzyme-linked Immunosorbent Assay (ELISA)

34. Estimation of pH and Preparation of Buffers

35. Biomedical Waste Management

36. DNA Isolation and Quantification

37. Biochemical Tests for Common Clinical Conditions

APPENDICES

1. Practical Biochemistry Question Bank
2. Multiple-choice Questions (MCQs) in Practical Biochemistry
3. III. Problem-solving Exercises, Interpretation of Laboratory Data and Case Studies in Practical Biochemistry
4. IV. Test Procedures for Qualitative and Quantitative Experiments in a Nutshell for Ready Reference

Index