The Book This book provides a concise introduction to qualitative and quantitative analysis of substances of biochemical importance for undergraduate medical students. It covers all the skill-based aspects of the CBME curriculum of biochemistry.
Special features of this edition
Rafi MD is the Dean and Medical Director of Surabhi Institute of Medical Sciences, Siddipet, Telangana.
A Mapping of the Contents to CBME Practical Biochemistry Curriculum Preface to the Fourth Edition Preface to the First Edition 1. Safety in the Laboratory Precautions First Aid in the Laboratory
QUALITATIVE EXPERIMENTS 2. Reactions of Carbohydrates Molisch’s Test Benedict’s Test Barfoed’s Test Seliwanoff ’s Test Foulger’s Test Rapid Furfural Test Osazone Test Acid Hydrolysis Methylamine Test for Lactose Mucic Acid Test for Galactose Bial’s Test for Pentoses Iodine Test for Polysaccharides Experiments Reactions of glucose Reactions of fructose Reactions of maltose Reactions of lactose Reactions of sucrose Identification of unknown carbohydrate 3. Reactions of Lipids Reactions of Triacylglycerols Reactions of Cholesterol 4. Reactions of Proteins Precipitation Reactions of Proteins Colour Reactions of Proteins Experiments Reactions of albumin Reactions of casein Reactions of gelatin Reactions of peptone Identification of unknown protein 5. Reactions of Non-protein Nitrogenous (NPN) Substances Urea Uric Acid Creatinine Identification of unknown NPN substance 6. Identification of Unknown Substance of Biochemical Importance Carbohydrates, Proteins and NPN Substances 7. Analysis of Urine Physical Examination Microscopic Examination Chemical Analysis 8. Analysis of Abnormal Constituents of Urine Glucose Ketone Bodies Proteins Blood Bile Salts Bile Pigments Identification of unknown abnormal constituents in urine 9. Qualitative Analysis of Saliva 10. Qualitative Analysis of Gastric Juice 11. Qualitative Analysis of Bile 12. Qualitative Analysis of Milk 13. Food Analysis
QUANTITATIVE EXPERIMENTS 14. Clinical Biochemistry Laboratory: Instrumentation and Techniques Glassware and Instruments Commonly Used in the Laboratory Solutions Sample Collection and Handling Colorimetry Spectrophotometer Autoanalyser Flame Photometer Fluorimeter Dry Chemistry Systems ABG Analysis Immunodiffusion Quality Control 15. Blood Glucose Sample Collection Determination of Glucose in Body Fluids 16. Glucose Tolerance Test Indications for GTT Glucose Tolerance Curve 17. Blood Urea and BUN Methods of Blood Urea Estimation Urinary Urea and Urea Clearance 18. Serum and Urinary Creatinine and Creatinine Clearance Creatine and Creatinine Creatinine Clearance 19. Uric Acid Formation and Excretion of Uric Acid Methods of Estimation 20. Serum Total Proteins and Albumin–Globulin Ratio 21. Cholesterol and Lipid Profile 22. Bilirubin Serum Bilirubin 23. Aminotransferases (AST and ALT or SGOT and SGPT) 24. Alkaline and Acid Phosphatases (ALP and ACP) Estimation of Alkaline Phosphatase Estimation of Acid Phosphatase 25. Creatine Kinase and Its Isoenzymes 25. Amylase and Lipase Estimation of Serum Amylase Estimation of Serum Lipase 26. Calcium and Phosphorus 26. Serum Electrolytes Electrolyte Analysis by Ion Selective Electrode 27. Quantitative Analysis of CSF 27. Electrophoresis Types of Electrophoresis 28. Chromatography 28. Spectroscopic Examination of Hemoglobin and Its Derivatives 29. Radioimmunoassay (RIA) and Enzyme-linked Immunosorbent Assay (ELISA) Radio Immunoassay (RIA) Enzyme-linked Immunosorbent Assay (ELISA) 30. Estimation of pH and Preparation of Buffers Determination of pH Using a pH Meter Buffers 31. Biomedical Waste Management Segregation of Waste Storage of Waste Transportation of Waste Treatment and Disposal of Waste Safety Measures 32. DNA Isolation and Quantification Basic Steps of DNA Isolation Quantification of DNA 33. Biochemical Tests for Common Clinical Conditions Diabetes Mellitus Dyslipidemia Myocardial Infarction Renal Failure Gout Proteinuria Nephrotic Syndrome Edema Jaundice Liver Disorders Pancreatitis Acid-Base Disorders Thyroid Disorders Vitamins and Minerals 34. Urinary Screening for Inborn Errors of Metabolism 35. Energy Content of Various Foods and the Significance of Glycemic Index Energy Requirements in Humans Energy Content of Foods Glycemic Index 36. Saturated, Unsaturated and Trans-Fatty Acids in Foods Saturated Fatty Acids Unsaturated Fatty Acids Biomedical Importance of PUFA Trans-fats APPENDICES I. Practical Biochemistry Question Bank Qualitative Experiments Quantitative Experiments II. Multiple-choice Questions (MCQs) in Practical Biochemistry III. Problem-solving Exercises, Interpretation of Laboratory Data and Case Studies in Practical Biochemistry IV. Test Procedures of Qualitative and Quantitative Experiments in a Nutshell Qualitative Test Procedures (in alphabetical order) Quantitative Test Procedures Spotters and Charts
Index